WebJan 15, 2024 · The HTRF E2 transthiolation assay was used to determine the IC 50 value of the UBA6 inhibitor against recombinantly purified UAE, NAE and SAE. This assay was run … WebJan 27, 2013 · A thioester assay was performed to determine the activity of the various cloned E2s. In the presence of E1, Ub is linked to active E2 proteins through thioester bonds. These thioester bonds are disrupted in the presence of …
High‐Throughput Assay to Monitor Formation of the …
WebIn vitro protein ubiquitination assay. Ubiquitination is one of the most important posttranslational modifications in all eukaryote organisms. Ubiquitin-activating enzyme … WebJan 28, 2024 · After biotinylation of C3b and C4b molecules at their thioester moiety, 1500 and 2100 response units (RUs), respectively, were immobilized onto a streptavidin SAP sensor chip. C3 was flowed over in concentrations ranging from 0.023 to 6 µM, C5 in concentrations from 0.011 to 3 µM. terrance taylor american football
An N-terminal p14ARF peptide blocks Mdm2-dependent ... - Nature
WebFeb 1, 2011 · A schematic overview of the acyl-RAC assay. Free thiols are first blocked with MMTS. Thioesters are then cleaved with neutral hydroxylamine (NH 2 OH), and the newly liberated thiols are captured with thiol-reactive Sepharose resin. WebJan 22, 2002 · Kinetic Assays of Peptide Thioester Substrates. TycC TE was expressed with a C-terminal hexahistidine tag and purified as described ( 6 ). Reactions were carried out … WebNational Center for Biotechnology Information terrance terry silver